Analysing the Effects of Copper Oxide Nanoparticles Incorporated Feed on the Growth and Haematology of Koi Carp
Chinnadurai Kaleeswaran
Department of Biology, The Gandhigram Rural Institute-Deemed to be University, Gandhigram 624302, India.
Murugeswaran Dayana Senthamarai
Department of Biology, The Gandhigram Rural Institute-Deemed to be University, Gandhigram 624302, India.
Muthuswami Ruby Rajan *
Department of Biology, The Gandhigram Rural Institute-Deemed to be University, Gandhigram 624302, India.
*Author to whom correspondence should be addressed.
Abstract
The objective of this study was to examine the effect of different concentrations of copper oxide nanoparticles (CuO NPs) incorporated feed on Koi carp growth and morphology. The CuO nanoparticles were synthesised and characterised by using various techniques, including UV-Visible Spectroscopy (UV-Vis), Scanning Electron Microscopy (SEM), Energy Dispersive X-Ray Spectroscopy (EDAX), Fourier Transform Infrared Spectroscopy (FT-IR), and X-ray Diffraction (XRD). To the 100 g of feed, various concentrations of CuO NPs, such as 0, 25, 50, 100, 150 and 200 mg/100 g, were incorporated. The Koi Carp’s haematological parameters and feed consumption were assessed after 21-day study duration. CuO NPs’ physical and chemical characteristics were examined. From UV-Vis analysis, CuO NPs exhibited a peak at 200–300 nm wavelengths, and FT-IR spectra exhibited the functional groups of CuO NPs in the range of 400 to 4000 cm–1. At resolutions ranging from 9.18 mm (scale bar 10 µm) to 9.18 mm (scale bar 2 µm), SEM images were observed. CuO NPs exhibited two peaks in the spectrum at 0.9 keV and 8 keV were detected by the EDAX spectrum. From the XRD analysis, the crystalline nature of CuO NPs was observed, and the crystalline size was 75 nm. In growth parameters of the Koi carp’s condition factor and feed utilisation metrics were greater in feed VI (mg). From feed I to feed VI, all other parameters are gradually reduced while the WBC count is steadily increased. This study concluded that Koi carp might grow well with 200 mg of copper oxide nanoparticles incorporated with feed.
Keywords: Copper oxide, incorporation, different quantities in feed, growth, haematology, quantities